Unbound unconjugated bilirubin present in serum of neonates instead of total serum bilirubin has been well established to provide a more reliable indicator to predict the toxicity of hyperbilirubinemia [1].Hemolysis has reported to be occured in neonates with G6PD deficiency [2],resulting in hyperbilirubinaemia in the newborn period [3].As the result of hemolysis,a lot of hemoglobin is released into the blood stream which can cause interference for the determination of free bilirubin under physiological pH.Two approaches are under investigation to remove the interference of excess hemoglobin.The first approach is removing hemoglobin prior to CE-frontal analysis and the second is separation of hemoglobin from bilirubin prior to determination under the CE-frontal analysis format.Results for the two approaches will be presented and their pros and cons discussed at the meeting.