【摘 要】
：
With the acceleration of proteome research, it becomes urgent to develop novel approaches for high efficient and high throughput analysis of proteins.2D nano-HPLC is one of the most prospective approa
【机 构】
：
National Chromatographic R&A Center, Dalian Institute of Chemical Physics, the Chinese Academy of Sc
【出 处】
：
第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
论文部分内容阅读
With the acceleration of proteome research, it becomes urgent to develop novel approaches for high efficient and high throughput analysis of proteins.2D nano-HPLC is one of the most prospective approaches, which provides high resolving power, versatility, sensitivity and automation.However, one of the greatest impediments to this method is low analysis throughput and time-consuming.To solve these problems, a high throughput 2D nano-LC/MS/MS platform with combination of strong cation exchange chromatography and C18 high-speed liquid chromatography column via dual capillary trap columns was established.
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