Solid surfaces in contact with biological cells play an important role in cell cultures.In vivo,most cells are embedded within an intricate extracellular matrix,which not only binds the cells and tissues together but also influences cellular development,polarity,and behavior.Recently,tissue engineering has attracted attention because of the potential of cell transplantation as an alternative therapeutic strategy for tissue repair and organ replacement.Transplanted cells cultured from healthy tissue of a patient can be implanted back into the patient without requiring an immunoisolation system.In cell cultures for tissue engineering,a matrix scaffold is necessary for adhesion of the cells,requiring a thorough study of potential substrates for the cell culture scaffolds.In this talk I present three unique types of solid surfaces,i.e.,super water-repellent surfaces,honeycomb polymer films,and thermo-responsive polymer-grafted glass surfaces.Preparation and physical properties are presented first followed by highlights of recent progress in cell culture on the solid surfaces (see H.Yan,et al.,Current Chemical Biology 2007,1,290).Furthermore,I also briefly present electrospun non-woven fabrics made of thermo-responsive nanofibers,which also may be used for scaffolds of cellular cultures.