【摘 要】
：
Although CRISPR/Cas9 system has been successfully applied to zebrafish studies,its efficiencies vary greatly for different gRNAs,even for those targeting on the same genes.As the workload and cost of
【机 构】
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同济大学生命科学与技术学院,上海200092
【出 处】
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第七届全国生物信息学与系统生物学学术大会
论文部分内容阅读
Although CRISPR/Cas9 system has been successfully applied to zebrafish studies,its efficiencies vary greatly for different gRNAs,even for those targeting on the same genes.As the workload and cost of zebrafish mutant screening is largely depended on the mutation ratio of injected embryos,selecting more effective gRNAs is especially important for zebrafish homozygous mutants construction.Besides the influence of sequence features,local chrornatin structures may have effects on CRISPR/Cas9 efficiency in zebrafish,which is largely unexplored.The only related study in zebrafish did not observe the effects of nucleosome organization on CRISPR/Cas9 efficiency,which is inconsistent with the recent in vitro study.
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