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The versatility of appendages in insects plays an important role in evolution and ecology.Development of these appendages is regulated by a complex set of genes including the Hox gene.To study the mechanism of Hox gene action on insect body plan,the E group of silkworm with more than 30 mutants is an excellent model.In silkworm,Abd-A that plays an important role in development of prolegs,and is expressed in most of the abdomen.In this study,we identified multiple novel alternative splice forms of Abd-A in E mutant silkworms.To investigate the contributions of Abd-A splice variants to prolegs development,we analyzed the expression levels of Abd-A mRNA and protein in E mutant Bombyx mori.In addition,we also analyzed the Distal-less (Dll) which is the downstream target gene of Abd-A,during embryogenesis.Further,we also performed a detailed analysis on the evolutionary pattern of the Abd-A alternative splice forms in diverse species.Together,our results suggest that (1) multiple spliceosomes may induce the over-expression of Abd-A and promote the expression of Dll for abdominal appendage development (2) successful evolution of Abd-A gene duplication and various splicing forms was adapted by organisms to perform complex and multiplex functions in vivo.