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Phytoplasmas are phloem-limited bacterial plant pathogens associated with approximately 1000 plant species worldwide, of which over 100 occur in China.In the study, four phytoplasma strains of chinaberry witches-broom (CWB) from Hainan, Guangdong and Fujian provinces (CWB-hnsy1,CWB-hnsy2, CWB-fjfz, CWB-gdgz), two strains of mulberry dwarf (MD) from Zhejiang and Anhui provinces (MD-ahhf and MD-zjca), two strains of lettuce yellow (LY) from Yongan, Fujian province (LY-fjsm1 and LY-fjsm2) and a strain of paulownia witches-broom (PaWB) from Shandong province,which were all identified as the member of 16SrI group phytoplasmas based on 16S rRNA gene sequences, were performed extensive sequence and phylogenetic analysis based on sequences of 16S-23S rRNA intergenic spacer region, partial 23S rRNA, ribosomal protein including 3end of S19 and complete L22 and S3 (rp), tuf , secA, secY, ipt , dnaK, fusA , gyrB , pyrG and rpoB gene sequence data from these nine phytoplasma strains, as well as onion yellows phytoplasma (OY-M), aster yellows witches-broom phytoplasma (AYWB) and Acholeplasma laidlawii PG-8A strains, which were employed as reference.We found abundant genetic variation in these 16SrI group phytoplasma strains.