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目的研究大豆苷元对脑缺血再灌注损伤的保护作用,并初步探讨其保护作用与炎症反应之间的关系。方法采用大鼠大脑中动脉栓塞制作局灶性脑缺血再灌注损伤模型,缺血后10 min,于舌下静脉给不同剂量的大豆苷元。再灌注24 h后,断头取脑,TTC染色,测定脑梗死体积;取前脑称湿重,烘干后称干重,计算脑组织含水率;取血2 ml,分离血清,放射免疫学方法检测血清IL-1β及TNF-α含量。结果与模型组相比,大豆苷元治疗组脑梗死范围减小,脑组织含水率显著降低(P<0.05),血清IL-1β及TNF-α含量显著降低(P<0.05)。结论大豆苷元对脑缺血再灌注损伤具有一定的保护作用,其保护机制与降低炎性细胞因子TNF-α,IL-1β的表达进而减轻脑水肿有关。
Objective To study the protective effect of daidzein on cerebral ischemia-reperfusion injury and to explore the relationship between its protective effect and inflammatory response. Methods A model of focal cerebral ischemia-reperfusion injury was established by middle cerebral artery occlusion in rats. Ten minutes after ischemia, different doses of daidzein were given to the sublingual vein. Twenty-four hours after reperfusion, the brain was decapitated and TTC stained to determine the volume of cerebral infarction. The wet weight of forebrain was taken and the dry weight was taken after drying. The water content of brain tissue was calculated. The blood was collected 2 ml, serum was separated and radioimmunoassay Methods The contents of IL-1β and TNF-α in serum were detected. Results Compared with the model group, the daidzein treatment group decreased the range of cerebral infarction, the brain water content decreased significantly (P <0.05), the serum IL-1β and TNF-α levels decreased significantly (P <0.05). Conclusion Daidzein has a protective effect on cerebral ischemia-reperfusion injury. Its protective mechanism is related to reducing the expression of inflammatory cytokines TNF-α, IL-1β and decreasing cerebral edema.