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Sparganosis is a serious food borne parasitic zoonosis caused by infection with Spirometra spargana.The prevalence of sparganum infection in wild frogs (Rana nigromaculata,R.limmochari,R.temporaria and Bufo gargarizans) was investigated in Henan Province of central China during 2008:2012.Out of 3482 caught wild frogs,565 (16.23%) frogs were found to be infected with plerocercoids (spargana) of the genus Spirometra.Spargana were found in 14.85% (320/2155) ofR.nigromaculata,20.82% (233/1119) ofR.limrmochari and 20.82% (233/1 119) ofR.temporaria frogs.However,no sparganum was found in B.gargarizans.To investigate the phylogenetic position of the collected spargana,three mitochondrial DNA (mtDNA) regions,namely cytochrome coxidase subunit 1 and 3 (cox 1 and cox3),NADH dehydrogenase subunits 4(nad4)were amplified,sequenced,and analyzed.The sequences ofcoxl,cox3 and pnad4 were 417,390 and 578 bp in length,respectively.The base composition ofcoxl,cox3 and pnad4 were generally AT rich with a mean of 63.5%,68.3% and 67%,respectively.Phylogenetic analysis showed that all the sparganum isolates in Henan province represented Spirometra erinaceieuropaei and were a well:supported clade.These findings demonstrated clearly the usefulness of the three mtD-NA sequences for molecular identification and population genetics studies ofS.erinaceieuropaei spargana of human and animal health significance.