The biodegradable temperature-and redox-responsive poly(ether urethane)s were synthesized for intracellular delivery of anticancer drug.The amphiphilic poly(ether urethane)s were comprised of 2,2-dithiodiethanol, hydrophobic hexamethylene diisocyanate and hydrophilic poly(ethylene glycol) (PEG) segments, which was used to prepare doxorubicin (DOX)-loaded poly(ether urethane) nanoparticles.The phase transition temperature (Tp) of poly(ether urethane)s in aqueous solution could be easily regulated by changing the ratio of PEG to 2,2-dithiodiethanol.The redox-responsive disulfide bonds in poly(ether urethane)s could be cleaved responding to glutathione (GSH) when the temperature was above Tp, while the degradation was inhibited below Tp.The nanoparticles exhibited a obvious redox-responsive release of DOX above Tp.Fluorescent imaging indicated that the nanoparticles could be effectively internalized into HepG2 cells, and the nanoparticles showed a high cytotoxicity against HepG2 cells, while the polymer itself had no significant toxicity.The temperature-and redox-responsive nanoparticles have a promising potential for intracellular delivery of anticancer drug.