【摘 要】
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A major assumption of the high-dose/refuge strategy proposed for effectively managing and/or delaying the resistance development in target insects for transgenic crops expressing Bacillus thuringiensi
【机 构】
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State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Ch
【出 处】
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The International Conference on Insect Molecular Ecology and
论文部分内容阅读
A major assumption of the high-dose/refuge strategy proposed for effectively managing and/or delaying the resistance development in target insects for transgenic crops expressing Bacillus thuringiensis(Bt)toxins is that the resistance is recessive or incompletely recessive.A population of Asian corn borer,Ostrinia furnacalis(Guenée),selected with Cry1Ie in the lab for several generations,had evolved 35-fold resistance to Cry1Ie.The inheritance of resistance to Cry1Ie in the resistant strain was determined through bioassay by exposing neonates of reciprocal crosses and backcross offspring to agar-free semi-artificial diet incorporated with Bt proteins.The response of progeny of reciprocal crosses between resistant and susceptible strains were not significantly different,indicating that the resistance was autosomal.At median lethal concentration,the dominance of resistance(D)was-0.72(susceptible female×resistant male)and-0.51(resistant female×susceptible male),indicating that resistance was incompletely recessive.However,the D decreased as the concentration increased.Bioassay of the backcross of the F1 generation with the selected strain suggested that the resistance to Cry1Ie was controlled by more than one locus.In addition,the resistant strain had no cross-resistance to Cry1Ab,Cry1Ac,Cry1F and Cry1Ah.The result not only offers the guide for managing the resistance development,but also suggests that Cry1Ie can be proposed as an appropriate candidate for expression with Cry1Ac or Cry1Ab for the development of Bt maize in China.
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