Adipose-Derived Stem Cells Improved Mouse Ovaryfunction after Chemotherapy-Induced Ovary Failure

来源 :2013首届世界健康大会 | 被引量 : 0次 | 上传用户:ktcalf
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  Introduction: Young patients receiving chemotherapy occasionally face infertility and premature ovarian failure (POF).Numerous investigations reported that adipose-derived stem cells (ADSCs) transplantation could amelioratethe structure and function of injured tissues.Inthis studywe explore the therapeutic efficacy of ADSCtransplantation for chemotherapy-induced ovarian damage.Methods: Female mice were injected intraperitoneally with cyclophosphamide (CTX).After 15consecutive days of injection, ADSCs were transplanted either directly into bilateral ovaries or via intravenousinjection, and the ovaries were excised after either 1 week or 1 month of treatment.The follicles were cotmted andcategorized, and ovarian histologic sections were stained for TUNEL.Ovarian function was evaluated by monitoringovulation.ADSC tracking, microarray analyses, and real-time polymerase chain reaction (PCR) were used to assessthe inner mechanism of injury and repair.Results: The ovarian function of mice exposed to CTX injection improved after ADSC transplantation.Thepopulation of follicles at different stages and ovulation significantly increased after the treatment.Immunofluorescence revealed reduced TUNEL staining.The tracking of ADSCs revealed that these cells did notdirectly differentiate into the follicle component.Microarray analyses indicated that changes in different groups ofgenes might affect follicle formation or ovulation.Conclusions: ADSC transplantation improved ovarian function.Our results suggest a potential mechanism forADSC therapy.
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