【摘 要】
:
Introduction: Young patients receiving chemotherapy occasionally face infertility and premature ovarian failure (POF).Numerous investigations reported that adipose-derived stem cells (ADSCs) transplan
【机 构】
:
Fourth Military Medical University China
论文部分内容阅读
Introduction: Young patients receiving chemotherapy occasionally face infertility and premature ovarian failure (POF).Numerous investigations reported that adipose-derived stem cells (ADSCs) transplantation could amelioratethe structure and function of injured tissues.Inthis studywe explore the therapeutic efficacy of ADSCtransplantation for chemotherapy-induced ovarian damage.Methods: Female mice were injected intraperitoneally with cyclophosphamide (CTX).After 15consecutive days of injection, ADSCs were transplanted either directly into bilateral ovaries or via intravenousinjection, and the ovaries were excised after either 1 week or 1 month of treatment.The follicles were cotmted andcategorized, and ovarian histologic sections were stained for TUNEL.Ovarian function was evaluated by monitoringovulation.ADSC tracking, microarray analyses, and real-time polymerase chain reaction (PCR) were used to assessthe inner mechanism of injury and repair.Results: The ovarian function of mice exposed to CTX injection improved after ADSC transplantation.Thepopulation of follicles at different stages and ovulation significantly increased after the treatment.Immunofluorescence revealed reduced TUNEL staining.The tracking of ADSCs revealed that these cells did notdirectly differentiate into the follicle component.Microarray analyses indicated that changes in different groups ofgenes might affect follicle formation or ovulation.Conclusions: ADSC transplantation improved ovarian function.Our results suggest a potential mechanism forADSC therapy.
其他文献
The regeneration and repair of articular cartilage after the evolution of four generation technology.The first generation of technology as the periosteum overlying cartilage defect, then inject amplif
Lgr5 has been approved to be the stem cell marker in mouse gastrointestinal tract.Evidences also show that Lgr5+ and Bmi1+ cells may represent two stem cell sub-populations with different functions in
The esbablishrnent of human embryonic stem cells, and lately, induced pluripotent stem cells (hESC, hiPSC, respectively) greatly expanded our view to subtly analyze the cellular and molecular happenin
Clostridium difficile (Cd) is a well known emerging pathogen of opportunistic infection in hospitals worldwide and is the major causative antibiotic-associated pseudo-membranous colitis and diarrhea i
The development of the stem cell therapy has been recognized for solving various rare and incurable diseases that modem medicine has not solved yet.Over the past 10 years of hard work and investment i
Recent studies have revealed that following injuries, soft tissues such as anterior cruciate ligaments (ACL), release large amounts of matrix metalloproteinases (MMPs).These enzymes have a devastating
In bone tissue engineering and regeneration we have generated a unique transgenic mouse line, in which luciferase and GFP reporters are driven by a bone specific gene promoter and an actin promoter, r
Although induced pluripotent stem cells (iPSCs) can be reprogrammed from human somatic cells using four defined factors, the process is extremely inefficient, as there is a strong epigenetic block pre
Objective: To investigate the efficiency ofautologous transplantation of peripheral blood stem cells for treatment of patients with diabetic lower limb ischemia.Methods: Eighteen patients of type 2 di
Emerging evidence indicates that human mesenchymal stem cells (hMSCs) can be recruited to tumor sites, and affect the growth and development of human malignancies.However, there is some controversy re