In order to explore the genetics of dark-induced senescence in winter wheat(Triticum aestivum L.), a quantitative trait loci (QTL) analysis was carried out in a doubled haploid population, developed from a cross between varieties Hanxuan 10 and Lumai 14.Chlorophyll content (Chl a+b, Chl a, and Chl b),original fluorescence (Fo), maximum fluorescence level (Frm), maximum photochemical efficiency (Fv/Fm), and ratio of variable fluorescence to original fluorescence (Fv/Fo) were evaluated in the second leaf of whole three-leaf seedlings subjected to 7 d of darkness.