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目的 To explore the role of PRIM1 in liver cancer 方法 Quantitative polymerase chain reaction(QPCR)was used to detect mRNA expression of PRIM1 in BEL-7404,BCL-7402,HepG2 and SMMC-7721 cell lines.LV-PRIM1-RNAi was transfected into BEL-7404 and SMMC-7721 cells by lentivirus,and then Celigo imaging cytometer,Caspase3/7 Assay,flow cytometry and MTT assay were used to detect the proliferation and apoptosis of BEL-7404 and SMMC-7721 cells with ≥50％gene reduction rate after lentivirus transfection detected by QPCR.BEL-7404 and SMMC-7721 carrying PRIM1 gene were used for oncogenesis in vitro to observe the weight and fluorescence intensity of the tumor 结果 QPCR results showed that PRIM1 was expressed in BEL-7404,BCL-7402,HepG2 and SMMC-7721 cell lines,which was highest in BCL-7404 cell line.Celigo imaging cytometer,Caspase3/7 Assay,flow cytometry and MTT assay showed that the proliferative ability of BEL-7404 and SMMC-7721 were decreased after LV-PRIM1-RNAi transfection.Furthermore,the weight and the fluorescence intensity of the tumors in vitro formed by LV-PRIM1-RNAi cells on SCID mice were decreased.Wnt5a may be a downstream target gene of PRIM1 结论 Interference of PRIM1 expression can inhibit the proliferation of BEL-7404 and SMMC-7721 cells,and induce the apoptosis of liver cancer cells.