【摘 要】
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Objective To find out which TLRs can be activatied by sporozoite and discuss its effort in development of malaria Liver-Stage.Methods Firstly, we acquired purified sporozoites through DE52 column.Then
【机 构】
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Department of Pathogenic Biology, The Third Military Medical University, Chongqing 400038, China
【出 处】
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中华医学会第六次全国艾滋病、病毒性丙型肝炎暨全国热带病学术会议
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Objective To find out which TLRs can be activatied by sporozoite and discuss its effort in development of malaria Liver-Stage.Methods Firstly, we acquired purified sporozoites through DE52 column.Then we amplified and cloned the full-coding sequence of mice TLR1, TLR3, TLRS, TLR6, TLR7 and TLR9, and established a TLRs agonist screening platform, then systemically screened the possible TLRs agnoists contained in sporozoites lysate using NF-κB or IFN-β reporter assay; Secondly, we tested whether sporozoites lysate could induce peritoneal macrophage to release inflammatory cytokines dependent on both TLR2; Finally, the inhibitory role of TLR2 in the development of pre-erythrocytic stage was determined according to the level of both liver parasite load and parasitemia in the wide-type and TLR2 knockout mice after challenged with wild-type sporozoites.
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