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Bisphenol A (BPA) among the groups of endocrine disrupting chemicals (EDCs) has aroused growing concerns because of their carcinogenicity and ubiquitousness.Herein, dispersive liquid-liquid microextraction (DLLME) with in situ derivatization was developed for determination of BPA in water samples by gas chromatography-mass spectrometry (GC-MS).An orthogonal design was employed to optimize the DLLME conditions and acetic anhydride was used for direct derivatization in aqueous media.