[Objective]The aim of this work was to study the molecular machanism of SigB in biofilm formation by Listeria monocytogenes.[Methods]Here,Listeria monocytogenes WaX12 and WaX12-△sigB biofilm was investigated using RNA-seq technology,and the differentially expressed genes were analyed using Cuffdiff.RNA-seq data was validated by examining the expression of six genes involved in biofilm formation by qRT-PCR.[Results]The results indicated that 701 significant difference expression genes between WaX12 and WaX12-△sigB biofilm,consisted of up-regulated genes (55.92 %),down-regulated genes (44.08 %).Analysis of GO database showed that all differentially expressed genes were classified to biological process (Ribosome biogenesis) and molecular function (structural constituent of ribosome).74 different KEGG pathways related to the role of sigB in biofilm formation of L.monocytogenes were investigated,and showed a significant correlation with Ribosome metabolic and Pentose and glucuronate interconversions metabolic.[Conclusion]These findings revealed that loss of sigB altered gene expression patterns in L.monocytogenes biofilm and resulted in reduced ability of the biofilm formation.This work will provide theoretical research foundation on efficiently preventing and controlling L.monocytogenes biofilm formation.