Replication factor C 1 (RFC1), conserved in eukaryotes, is involved in DNA replication and checkpoint control.However, a RFC1 product participating in DNA repair at meiosis has not been reported in Arabidopsis.Here, we report a functional characterization of AtRFC1 through the analysis of the rfc1-2 allele.The rfc1-2 mutant displayed normal vegetative growth but had silique sterility because the male gametophyte was arrested at the uninucleus microspore stage and the female at the functional megaspore stage.