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Analysis of drug concentration in serum/plasma with high performance liquid chromatography (HPLC) is widely used in both overall drug development process and therapeutic drug monitoring (TDM).Commonly used HPLC is ordinarily a single channel system consists of one pump and one column with auto-sampler, detector and working station.Biological samples must be pretreated before injection into the system.Conventional sample preparation techniques include liquid-liquid extraction (LLE), solid-phase extrction (SPE) and protein precipitation (PP).The former two techniques are always labour intensive, low detection through-put and error prone processes with multi-step manual operations.PP is a simple and easy handling technique, but the supernatant is always impure with large volumn.