A comparative proteomics study on matrix vesicles of osteoblast-like Saos-2 and U2-OS cells

来源 :第八届全国医学生物化学与分子生物学、第五届全国临床应用生物化学与分子生物学、2013华东六省一市生物化学与分子生物学联合 | 被引量 : 0次 | 上传用户:beiebi3807b
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
  Matrix vesicles (MVs) play an important role in the initial stage of the process of bone mineralization, and are involved in multiple rare skeletal diseases with pathological mineralization or calcification.
其他文献
进行性骨化性纤维发育不良(Fibrodysplasia ossificans progressiva, FOP)是一种罕见的与遗传密切相关的全身性进行性结缔组织病,发生部位通常位于一些结缔组织,如骨骼肌、韧带、跟腱等,全球发病率约为二百万分之一,其典型特征就是先天性大趾畸形和进行性异位骨化.
In normal and healthy individuals, bone formation is normally limited to the specialized tissues, the skeleton and the dentition which forms through endochondral ossification or intramembranous ossifi
目的:构建E731KFGFR2-3 ×FlagCMV真核表达载体,为研究E731K FGFR2突变基因的功能奠定基础。方法:采用定点突变试剂盒对正常人源FGFR2基因进行定点突变,携带有XhoI和XbaI酶切位点的PCR引物扩增目的基因,分别对胶回收PCR产物和3×FlagCMV表达载体进行双酶切,并切胶回收,T4DNA连接酶16℃连接过夜,转化感受态大肠杆菌DH5α,挑选克隆并进行菌落PCR,挑
MicroRNAs (miRNAs) are a kind of noncoding RNA of approximately 22 nucleotides which can bind to the untranslated region (UTR) of target mRNA and regulate the stability and translation of mRNAs, resul
成骨不全症(Osteogenesis imperfecta,OI)是一种以结缔组织紊乱为特征的罕见遗传性疾病,其中常染色体显形遗传的Ⅴ型OI约占5%,系IFITM5基因c.-14C>T突变所致.其典型的临床表现为前臂旋转严重受限,骨折或矫正手术后产生增生性骨痂,骨间膜钙化.
目的:就目前文献已报道的10个常染色体显性和隐性基因对患者进行突变检测,以发现所研究患者在这些致病基因上的突变位点。方法:采集成骨不全患者血液标本并搜集临床资料;提取全血基因组DNA;针对文献报道的所有成骨不全致病基因设计引物,做PCR扩增反应;直接测序法获得患者DNA序列;用Chromas,Mutation Surveyor等测序软件分析突变结果;通过对比成骨不全数据库,以发现新的突变。
成骨不全症(Osteogenesis imperfecta,OI)是一种由于胶原代谢异常引起的遗传代谢性疾病,约90%的患者由于Ⅰ型胶原编码基因COL1A1或COL1A2的突变引起,而人体成纤维、成骨细胞均可合成大量Ⅰ型胶原。的酶活性的测定方法等。通过这些方法的汇总以期为成骨不全症等胶原代谢性疾病的研究提供参考。
the ultra-weak light emission(UPE) is a general phenomenon of living systems, exists in a variety of animals, plants, algae and microbial systems, and is a inherent function of organism.
Matrix vesicles (MVs) involved in the initiation of mineralization by deposition of hydroxyapatite (HA) in their lumen are released by the budding of mineralization-competent cells during skeletogenes
The aim of this study was to investigate two mineralization-related genes TNAP and ANKH polymorphisms associated with ankylosing spondylitis (AS) in the North Chinese Han population.