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RAPD technique was applied to analyze the genetic diversity of Monilinia fructicola isolates from Beijing (B J), Shandong (SD), Hubei (HB), Zhejiang (ZJ), Fujian (FJ) and Yunnan (YN) provinces in China.Diversity was low among isolates of geographical populations, but high between populations.SD isolates were genetically closest to HB isolates;FJ isolates were genetically most distant.PCR and sequence analysis revealed all isolates contained a 1166 bp in length intron located just downstream of the G143 position of the cytochrome b (Cyt b) gene.These results indicate that the 1166-bp intron is present in genetically diverse M.fructicola populations from China and confirm our hypothesis that the G143A mutation conferring high levels of QoI resistance may not develop in M.fructicola.