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目的:观察黄斑明目颗粒对大鼠光化学损伤后视网膜的保护作用。方法:60只SD大鼠随机分为正常对照组、模型组、黄斑明目颗粒组。除正常对照组外,各组大鼠接受(1 900±106.9)Lux绿色荧光灯24 h持续光照射,建立大鼠视网膜光化学损伤模型。黄斑明目颗粒组于光照前7天分别予以黄斑明目颗粒2.5 g/(kg/d)灌胃给药,正常对照组及模型组予以等量生理盐水。光照后6、6、14 d,检测视网膜组织中超氧化物歧化酶(SOD)的活性及丙二醛(MDA)的含量,并进行视网膜透视电子显微镜和光学显微镜的组织学观察。结果:光化学损伤后6 d及14 d,黄斑明目颗粒组视网膜中SOD的活性显著高于模型组(P<0.05),MDA的含量显著低于模型组(P<0.05);通过视网膜透视电子显微镜和光学显微镜的组织学观察,黄斑明目颗粒组的病理组织学损害较模型组明显减轻。结论:黄斑明目颗粒对视网膜光化学损伤有明显的防护作用。
OBJECTIVE: To observe the protective effect of Huangmaimingmu Granule on the retina after photochemical injury in rats. Methods: Sixty Sprague-Dawley rats were randomly divided into normal control group, model group and macular spots group. Except for the normal control group, rats in each group were exposed to (19 900 ± 106.9) Lux green fluorescent light for 24 hours to establish a photochemical injury model of the retina in rats. The macular eyesight granule group was administrated macular eyesight granules 2.5 g / (kg / d) 7 days before light irradiation, while the normal control group and the model group were given the same amount of normal saline. The activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in retinal tissue were detected at 6, 6 and 14 days after light exposure, and the histological observations were made by electron micrograph and optical microscope. Results: At 6 and 14 days after photochemical injury, the activity of SOD in retina of Huangmen Mingmu Granule Group was significantly higher than that in model group (P <0.05), and the content of MDA was significantly lower than that in model group (P <0.05) Microscopy and optical microscopy of histological observation, macular hole group of pathological tissue damage than the model group was significantly reduced. Conclusion: Huangma Mingmu Granule has a significant protective effect on retinal photochemical damage.