【摘 要】
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Trichinella spiralis surface proteins are directly exposed to the hosts immune system and may play an important role in the larval invasion and development process.The analysis and characterization of
【机 构】
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Department of Parasitology,Medical College,Zhengzhou University,Zhengzhou 450052,China
【出 处】
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中国畜牧兽医学会家畜寄生虫学分会第七次代表大会暨第十二次学术研讨会
论文部分内容阅读
Trichinella spiralis surface proteins are directly exposed to the hosts immune system and may play an important role in the larval invasion and development process.The analysis and characterization of T.spiralis larval surface proteins could provide attractive information to identify the invasion:related proteins,the early diagnostic antigens and the targets for vaccine.The purpose of this study was to analyze the surface proteins of T.spiralis muscle larvae by two:dimensional gel electrophoresis (2:DE) and mass spectrometry.The 2:DE results showed that a total of approximately 33 proteins spots were detected with isoelectric point (pⅠ) varying from 4 to 7 and molecular weight from 10 to 66 kDa.Thirty:three protein spots were selected and identified by MALDI: TOF/TOF:MS analysis.Twenty:seven of 33 protein spots were identified and characterized to correlate with 15 different proteins.Out of the 14 protein identified as T.spiralis proteins,5 proteins [partial P49 antigen,deoxyribonuclease Ⅱ family protein,serine protease (gi| 168805931),serine protease (gi| 168805933) and serine proteinase] had catalytic and hydrolase activity.All the 5 proteins were also related to metabolic process and 2 of five proteins related to cellular process.The protein profile of T.spiralis surface proteins provides a valuable basis for further studies of the invasion:related proteins and the early diagnostic antigens for trichinellosis.
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