Giant pandas(Ailuropoda melanoleuca)and red pandas(Ailurus fulgens),two precious and endangered animals for wildlife conservation(Yonzon and Hunter,1991;Peng et al.,2001),are respectively classified as Category Ⅰ and Ⅱ protected species in China.Pathogen infections have been identified as the most serious threat for survival of wild pandas in China currently(He et al.,2012).Enterocytozoon bieneusi is an emerging and opportunistic enteric pathogen triggering diarrhea and enteric disease in humans and animals.It has been detected in immune-competent individuals,resulting in self-limiting diarrhea(Nkinin et al.,2007).More importantly,it is considered as an opportunistic pathogen which can cause life-threatening diarrhea in immune-compromised individuals especially in AIDS patients,raising public health concerns(Li et al.,2015).