【摘 要】
:
采用高效离子交换色谱和紫外检测系统建立快速分离纯化青枯雷尔氏菌的细菌色谱方法.通过比较青枯雷尔氏菌悬浮在哌嗪-HCL缓冲体系和双蒸水中后菌体数变化及细胞形态变化,分析该缓冲液对青枯雷尔氏菌生长活性及细胞表面特性的影响.结果 表明,青枯雷尔氏菌悬浮在平衡缓冲液(0.02 mol/L哌嗪-HCL)、洗脱缓冲液(0.02 mol/L哌嗪-HCl+1 mol/L NaCl)和双蒸水中其菌体数量无明显差异,
【机 构】
:
福建省农业科学院农业生物资源研究所,福州350003
【出 处】
:
第八届全国微生物资源学术暨国家微生物资源平台运行服务研讨会
论文部分内容阅读
采用高效离子交换色谱和紫外检测系统建立快速分离纯化青枯雷尔氏菌的细菌色谱方法.通过比较青枯雷尔氏菌悬浮在哌嗪-HCL缓冲体系和双蒸水中后菌体数变化及细胞形态变化,分析该缓冲液对青枯雷尔氏菌生长活性及细胞表面特性的影响.结果 表明,青枯雷尔氏菌悬浮在平衡缓冲液(0.02 mol/L哌嗪-HCL)、洗脱缓冲液(0.02 mol/L哌嗪-HCl+1 mol/L NaCl)和双蒸水中其菌体数量无明显差异,分别为4.8×108cfu/ml、4.0×108cfu/ml和5.5×108cfu/ml,透射电镜观察发现,3种溶液处理后,青枯雷尔氏菌均保持完整的细胞结构.研究了缓冲液pH值、流速及菌体细胞浓度对青枯雷尔氏菌色谱分离纯化效果的影响,确定青枯雷尔氏菌的最佳色谱分离纯化条件为:缓冲液pH值为8.0,流速为2mL/min,菌体浓度要大于108数量级而小于1011数量级.
其他文献
棉酚是存在于棉籽粕中的一种对动物有害的物质,因此若将棉籽粕作为动物饲料蛋白源,其利用率会大大提高(不足30%).本研究在失活的热带假丝酵母原生质体与未失活的酿酒酵母原生质体,在35%的聚乙二醇(PEG6000.A.R)和0.1MCa*诱导下融合,形成融合子.经培养鉴定,失活的热带假丝酵母代谢淀粉的糖化酶系统,在融合子中被未失活的酿酒酵母原生质酶系激活,以淀粉培养基为筛选培养筛选得到融合子.采用间苯
海鞘是目前国际上发现天然产物最多的海洋动物之一。近些年有直接或间接证据表明,从海鞘中分离得到的部分具有生物活性的天然产物其真正生产者不是海鞘本身,而是海鞘体内的微生物。威海海域海鞘资源丰富,不同种类海鞘来源的天然产物和海洋微生物有很大的差别,因此对威海海域海鞘及其微生物资源进行系统的开发和利用是发现新天然产物的有利途径。
Tibetan kefir grains are unique and valuable natural starter cultures for milk fermentation in Tibetan plateau,which contain a complex symbiotic diversity of microorganisms,mainly lactic acid bacteria
Previous studies suggested a close relationship between ruminant gut microbes and the mammary gland.In this study,shotgun metagenomic sequencing was used to reveal the differences in the intestinal mi
[Background]the diversity and the capacity to produce bioactive products of marine actinomycetes in the remote Arctic Ocean have seldom been studied.[Approaches]Both culture-independent and-lependent
Lactobacillus fermentum is economically important in the production and preservation of fermented foods.A repeatable and discriminative typing method was devised to characterize L.fermentum at the mol
The class Actinobacteria has been a goldmine for the discovery of antibiotics and has attracted interest from both academics and industries.However,an absence of novel approaches during the last few d
Objective: The objective of this study was to investigate effect of microencapsulated Lactobacillus plantarum LIP-1 on gut microbiota in a hyperlipidemic rat model,and to discuss the associations betw
Lactic acid bacteria that can produce alpha-galactosidase are a promising solution for improving the nutritional value of soy-derived products.For their commercial use in the manufacturing process,it
The objective of this study was to evaluate the influence of 9 Lactobacillus (L.)plantarum with broad-spectrum antibacterial activity on fermented milk,including changes to the fermentation characteri