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1 Introduction Eggs with a large proportion of thick white is desirable for consumers.The ability of hens to secrete a greater amount of thick albumen in the eggs was demonstrate to be an inherited and individual characteristic.With the generation and development of next generation sequencing(NGS)technology,identifying genes underlying egg albumen traits and incorporating them into genetic evaluation systems would be valuable for poultry breeding programs.2 Materials and Methods A total of 404 hens for Rhode Island white were used as the experimental populations.Eggs were collected in three successive days to measure relative egg composition traits at 30,40,and 50 weeks of age.At the age of 50 week,ten females were used for slaughtering after laying,from two groups:the extreme high thick albumen(HTA:n = 5),and the extreme low thick albumen(LTA:n = 5),representing chickens with high and low thick albumen eggs,respectively.RNA-Seq was used to profile the magnum tissue transcriptome and identify differentially expressed genes.GO and KEGG analysis were carried out to define the biological functions of these genes.Genes with a P-value <0.05 and absolute value of log2(fold_change)≥ 1 were assigned as differehtially expressed genes.3 Results A total of 220 genes were differentially expressed between the two groups,in which 150 genes were up-reg-ulated and 70 genes were down-regulated in high thick albumen(HTA)compared with low thick albumen(LTA)groups.Gene Ontology(GO)analysis revealed that genes up-regulated in HTA were involved mainly in bi-ological process.Based on the functional analysis of DEGs,ST3GAL4,FUT4,ITGA2,SDC3,PRLR,CDH4,GALNT9 were identified as promising candidate genes for thick albumen synthesis,transport and metabolism dur-ing the formation of egg laying.4 Discussion and Conclusion The related genes have been reported to participate in the regulation of oviduct-specific glycoprotein and other oviduct-specific proteins,have their effects on the forming of egg white.These novel insights into metabol-ic transcriptome of chicken magnum tissues will be valuable resources for development of strategies to optimize the proportion of thick egg albumen.