【摘 要】
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The clinical significance of ADAMs (a disintegrin and a metalloprotease) is evidenced by their implication in pathological process, including cancer, inflammation,Alzheimers disease and rheumatoid art
【机 构】
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Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Shanxi, xi'an,71
【出 处】
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Iternational Frontiers Symposium foe Neuron & Disease(国际神经与疾
论文部分内容阅读
The clinical significance of ADAMs (a disintegrin and a metalloprotease) is evidenced by their implication in pathological process, including cancer, inflammation,Alzheimers disease and rheumatoid arthritis.In never system, one hand ADAMs regulates adhesion and migration of neuron as well as axonal growth.another hand ADAMs has a tight relationship with plasticity of activation of glia cell ,neurogenesis and synaptic remodeling.To investigate differential expression of ADAM family in DRG of rats after never injury, ten members of the ADAM8-12, ADAM15, ADAM17, ADAM19, ADAM22,ADAM23.Sixty adult male SD rats ,weighing 180~220g.Spinal nerve injury(SNI)was induced by exposure of sciatic nerve and its three branches and ligation and transaction of tibial and common fibular nerves, , while preserving the sural nerve intact.Thirty rats were randomly divided into 5 groups(n=12 each) according to control and different time course after SNI.2, 7, 14, 28d after opration, rats were killed, and The dorsal root ganglions (DRGs) of the lumbar segment(L4-5)were removed.Taking advantage of Trizol kit and RT-PCR kit, to extract total mRNA and reverse transcript to cDNA ,Using Primer5.0 to design primers of ten of the ADAM family and GAPDH, these were synthesized by shanghai sangon company.To measure the expression of ADAMs mRNA by PCR.Comparing with controls, mRNA of ADAM8, ADAM9, ADAM 10, ADAM 15 and ADAM 17 all upregulate after SNI, in particular ADAM 10, ADAM 15 and ADAM 17 increase significantly(p<0.05);ADAM 11 and ADAM23 decrease notably(p<0.05);while ADAM12, ADAM19 and ADAM22 show no difference between control and SNI.The expressions of ADAM10, ADAM11, ADAM15, ADAM17 and ADAM23 mRNA in a rat model of neuropathic pain show notable changes, maybe ADAMs family contributes to the development of neuropathic pain.
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