【摘 要】
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Gibberella stalk rot is a devastating soil-bome disease in maize,caused by the necrotrophic fungus Fusarium graminearum,and poses a grave threat to maize production and kernel quality worldwide.Maize
【机 构】
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National Maize Improvement Center of China,China Agricultural University,Beijing,China,100193
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Gibberella stalk rot is a devastating soil-bome disease in maize,caused by the necrotrophic fungus Fusarium graminearum,and poses a grave threat to maize production and kernel quality worldwide.Maize resistance to Gibberella stalk rot is a typical quantitatively-inherited trait,predominantly conditioned by a major resistance QTL-qRfg1 on chromosome 10.Through both high-resolution and association mappings,we resolved qRfg1 to the presence/absence variation related to a CACTA-like transposable element.An active gene ZmCCT,encoding a transcription factor with a single CCT domain,was found~2.5kb downstream of the transposon insertion.Transgenic complementation demonstrated that ZmCCT was crucial for the quantitative resistance to Gibberella stalk rot in maize.The transposon insertion leads to the spreading of DNA methylation to the ZmCCT promoter region,which results in the reduced gene expression after pathogen challenge and finally impairs the disease resistance of maize.
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