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HPLC was used to separate chiral compouds with chiral column.Another method was used in HPLC with chiral mobile phase additives to separate chiral compounds.The structure of the derivative takes an important role in separation.We derivatized 2-heptamine with benzene sulfonyl chloride and dansyl chloride, Using cyclodextrin derivatives as chiral mobile phase additives, 2-heptamine was separated by HPLC.