【摘 要】
:
Rice plants have evolved a series of coordinated defense responses rendering them inaccessible to most would-be herbivores, but little is known about how it defends itself against herbivore attack.The
【机 构】
:
State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Ministry of Ch
【出 处】
:
中国植物病理学会2014年学术年会
论文部分内容阅读
Rice plants have evolved a series of coordinated defense responses rendering them inaccessible to most would-be herbivores, but little is known about how it defends itself against herbivore attack.The small brown planthopper (SBPH;Laodelphax striatellus Fallen) is one of the most destructive insect pests of rice, little to nothing is known about the molecular mechanism of SBPH-resistance in rice.Here, a SBPH-resistant introgression line (Pf9279-4) and its SBPH-susceptible recurrent parent (02428) were assessed for understanding about how rice plants respond to herbivore attack at the molecular level.After determination the exact time at which protein expression peaked after infestation by SBPH via two-dimensional fluorescence difference gel electrophoresis analysis, an Affymetrix genome array was then used to profile global gene expression changes under SBPH infestation.Surprisingly, two MA-biosynthetic pathway genes showed reverse differentially expression change between SBPH-resistant introgression line (Pf9279-4) and its SBPH-susceptible recurrent parent (02428) before and after attack by SBPH, which prompted us to investigate the differential expression changes of iron (Fe) homeostasis related genes in rice plants during SBPH attack.Fortunately, the results of this study revealed that Fe homeostasis involved in rice plants resistance to SBPH.Identification of redox-active Fe as a trigger in rice plant resistance to SBPH exposes a new layer of plant defense.The data presented here provides valuable insights into how rice plants respond to herbivore attack in Fe homeostasis-mediated redox changes manner and provides a conceptual framework for an initial step in a comprehensive understanding of Fe homeostasis functions in rice plants resistance to herbivore attack.
其他文献
Magnaporthe oryzae, the causal agent of rice blast, has been used as a model for investigating the interaction between phytopathogenic fungi and plants.Identification genes involved in mycelial growth
稻瘟病菌(Magnaporthe oryzae)是研究植物宿主与病原菌之间相互作用关系的一种模式真菌,其主要侵染水稻,造成大量减产.Chm1是一类丝氨酸/苏氨酸蛋白激酶PAK家族的一员,PAKs通常作为Rho GTP酶的效应蛋白起作用.研究证明,酵母中Chm1同源基因Ste20与Cdc42互作,共同参与调控菌丝的生长和信息素反应等.稻瘟病菌中MoChm1通过一个高度保守的P21结合区域(PBD d
Leaf blight is caused by Phomopsis obscurans.The fungus can infect foliage, runners,petioles, but it cant infect fruit or root.Leaf blight is commonly found on plants after harvest.Leaf lesions start
稻曲病近年来在我国多个水稻产区已逐渐上升为主要病害,认识与防控稻曲病对国家粮食生产安全十分重要。稻曲病的突出特征是稻曲病菌特异性侵染水稻颖花形成体积数倍于稻粒的稻曲球,然而目前国内外对该过程的内在机理仍不清楚。由于稻曲病菌侵染进程会因水稻材料及环境条件的差异而改变,因此,我们结合显微镜和转录组学技术探索了稻曲病菌侵染水稻花器的分子机理。结果显示,在我们的实验条件下,用感病材料蒲江6号人工接种稻曲病
近年来内蒙古中东部地区盐碱地大面积开发种植水稻。水稻育苗是水稻生产的一个关键环节,直接关系到水稻的产量问题。目前内蒙古水稻生产还未发展到工厂化水平,秧苗培育是庭院式拱棚技术。在这样的农业种植区每年都有水稻立枯病的爆发,给水稻生产带来严重经济损失。水稻立枯病是寒地水稻常见的苗期病害,发生此类病害后,茎部发生枯萎,植株矮化、变黄发生轻时,苗床秧苗变黄,严重发生时会引起秧苗成片枯死甚至全床毁苗。有关水稻
稻曲病是由稻曲病菌(Ustilaginoidea virens)侵染水稻花丝造成的一种水稻穗部真菌病害,典型症状是形成外部具有大量厚垣孢子的稻曲球。在一定环境条件下,稻曲球的表面可形成一至数个菌核。本实验室最近从越冬、萌发特性和条件子囊孢子捕捉的分析表明,菌核萌发产生的子囊饱子可能是主要的初侵染源,在病害循环中起重要作用。因此,针对菌核和子囊孢子的控制是稻曲病防治技术的关键环节。目前,国内外主要采
禾谷孢囊线虫(Cereal Cyst Nematode,CCN)是小麦生产上的一种病原线虫,我国发生危害的是禾谷孢囊线虫(Heterodera avenae)和菲利普孢囊线虫(H.filipjevi).本研究的目的是利用南澳发展研究所(South Australian Research and Development Institute,SARDI)开发的土壤DNA病害检测技术-PreDicta
大豆孢囊线虫Heterodera glycines是大豆上重要病源线虫,而有关大豆孢囊线虫不同生理小种与寄主互作机理差异的研究仍十分缺乏。本研究通过分析大豆孢囊线虫3号与4号生理小种同大豆高感品种中黄13互作阶段的转录水平差异来探讨3号与4号生理小种对大豆寄主中黄13的应答差异。最终,应用有限的线虫材料通过抑制性消减杂交技术分别构建了大豆孢囊线虫侵染后二龄、三龄和四龄雌虫这3个龄期共6个cDNA抑
The fungus Pochonia chlamydosporia is one of the most promising biocontrol agents for some most important nematodes.It is easily cultivated in vitro and produces chlamydospores, which are resistant re
Rice blast disease, caused by the ascomycete fungal pathogen M.grisea (Hebert Barr, is a world-widely problem in rice growth and production.The rice blast system is a classical genefor-gene system, an