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Objective: To enhance the role targeting, design to link NGR sequence with tumstatin active peptides-T7s C-terminal, the derivant called T7-NGR.Methods: The cloning vector pMD-T7 and pMD-T7N were constructed by PCR and gene synthesis methods respectively, identificated by digestion and DNA sequencing.After the digested plasmids were isolated by the low melting point agarose electrophoresis, the target-fragment was cut off and mixed with the recovery of the digested vector pET28a.Expression vector pET-T7 and pET-T7N were constructed in low melting point agarose, identificated by digestion and DNA sequencing, transformed into competent E.coli BL21 (DE3), induced by IPTG.Result: Identification result shows that pET-T7 and pETT7N were correct.Tricine-SDS-PAGE results showed that IPTG concentration of 1 mM, after the induction of 25 ℃, 8h, T7 peptides and T7-NGR peptides have achieved the optimum conditions of expression.Conclusion:Has been successfully constructed the expression vectors of the two peptides, and got product, no coverage at home and abroad, laid the foundation for further activity experiments.