论文部分内容阅读
Optimization of ovum pick up (OPU) followed by in vitro embryo production (IVP) is strongly driven by the needs of both beef and dairy cattle breeders to enhance genetic improvement.The in vitro culture of presumed zygotes derived from single cow OPU is important for the production of quality blastocysts maintaining pedigree.The rapidly growing use of genomic selection in cattle has increased the interest in using OPU-IVP technology to increase the number of embryos and offspring per donor, thus allowing enhanced selection intensity for the next generation.The aim of this study was to compare the in-vitro developmental ability of both slaughterhouse-and OPU-derived oocytes.Cumulus oocyte complexes (COCs) were collected from cows twice a week using the ultra-sound guided OPU technique and from slaughterhouse ovaries.The cow COCs from OPU and slaughterhouse ovaries were matured respectively in IVM mediun for 24h and fertilized in IVF medium with thawed bulls sperm for 24 h and cultured in IVC medium for 7 days with Charles Rosenkrans medium (CR1) culture system.We have investigated developmental ability of oocyte from OPU and slaughterhouse ovaries depending on the oocyte maturation rate, cleavage rate and blastocyst rate.Results show that the cleavage rate (68% vs.55%) and blastocyst rate (35% vs.26%) in OPU-derived oocyte significantly higher than that in slaughterhouse ovaries (P<0.01).