Polymer vesicles can be served as a nice model cell in field of soft materials and biological cell research field, while they are also strong candidates in many application developments, such as drug delivering and so on.There have be plenty of research in this filed.Here we present some of our new and interesting results on this topic using our self-built Laser-trapping Raman Spectroscopy (LTRS) system.With this instrument, we can collect information of both morphology and Raman signal on a well-defined tiny area of a single cell.By modulating solution environment, such as PH value, we can also get to helpful understand about cell mediated response and material transport through cell membrane.