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Capillary electrochromatography (CEC) combines the best features of capillary electrophoresis (CE)and high performance liquid chromatography (HPLC): high separation efficiency of CE and the versatileselectivity and large sample capacity of HPLC. However,in practice,when CEC was used without pressure,often on a commercial CE instrument,there were problems and difficulties associated with bubblesformation and column dry-out. These problems can be solved by a pressurized CEC (pCEC) system,inwhich a mobile phase is driven by both a pressurized flow and an electroosmotic flow (EOF).