Myocardial infarction is a major cause of mortality worldwide.After myocardial infarction, the resulting ischemia would damage myocardial cells and initiate the rebuilding process of extracellular matrix (ECM), which is known as cardiac fibrosis.Cardiac fibrosis is the main reason for heart failure after myocardial infarction, and it is mainly conducted by cardiac fibroblasts.Although emerging reports indicate that cell-cell interaction is important to the cardiac fibrosis procedure, to our knowledge, few in vitro disease models considered about it.