Resistance of non-transgenic papayas to Papaya ringspot virus (PRSV) mediated by intron-containing h

来源 :2014年度中国热带作物学会遗传育种专业委员会/热带香料饮料作物专业委员会年会暨学术研讨会 | 被引量 : 0次 | 上传用户：birdobird

【摘要】

：

　　RNA-mediated virus resistance based on natural antiviral RNA silencing has been exploited as a powerful tool for engineering virus resistance in plants.In t

【作者】

：

W.SHEN G.YANG Y.CHEN P.YAN D.TUO X.LI P.ZHOU

【机构】

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KeyLaboratoryofBiologyandGeneticResourcesofTropicalCrops,MinistryofAgriculture,InstituteofTropicalBi

【出处】

：

2014年度中国热带作物学会遗传育种专业委员会/热带香料饮料作物专业委员会年会暨学术研讨会

【发表日期】

：

2014年10期

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　　RNA-mediated virus resistance based on natural antiviral RNA silencing has been exploited as a powerful tool for engineering virus resistance in plants.In this study, a conserved 3′-region (positions 9839-10117, 279 nt) of the capsid protein (CP) gene of Papaya ringspot virus (PRSV), designated CP279, was used to generate an intron-containing hairpin RNA (ihpRNA) construct by one-step, zero-background ligation-independent cloning (OZ-LIC).The RNaseⅢ-deficient Escherichia coli strain M-JM1091acY was identified as the best choice for producing large quantities of specific ihpRNA-CP279.

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Resistance of non-transgenic papayas to Papaya ringspot virus (PRSV) mediated by intron-containing h

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