目的:研究不同取代羧甲基壳聚糖(CMC)的生物降解性。方法:通过体外酶解试验,用粘度测定法研究了在溶菌酶作用下 CMC 的分子量。结果:溶菌酶作为催化 CMC 降解的非专一性酶,能有效地催化 CMC降解;酶解反应程度与酶浓度成线性关系;随着羧甲基壳聚糖取代度的增加,CMC 的降解速度加快。结论:通过控制取代度,可以得到不同生物降解要求的羧甲基壳聚糖。 Objective: To study the biodegradability of different substituted carboxymethyl chitosan (CMC). Methods: The molecular weight of CMC under the action of lysozyme was studied by in vitro enzymatic hydrolysis assay. Results: Lysozyme, a non-specific enzyme catalyzing the degradation of CMC, could effectively catalyze the degradation of CMC. The degree of enzymatic hydrolysis was linear with the concentration of the enzyme. With the increase of the degree of substitution of carboxymethyl chitosan, the degradation rate of CMC accelerate. Conclusion: Carboxymethyl chitosan with different biodegradability can be obtained by controlling the degree of substitution.