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A microsporidian was isolated from Papilio machaon Linnaeus, an insect of Lepidoptera.The complete sequences of rDNA is 4286bp, which has a 98% similarity with Nosema bombycis.The spores of this isolate from P machaon can infect silkworm.The phylogenetic trees ofrRNA genes show that it belong to Nosema genus, and was a sister to the clade comprising N.bombycis, Nosema spodopterae,and Nosema sp.PX1.