【摘 要】
：
EBV is one of the most highly transforming viruses known.The EBV-immortalized B-cells use a viral program, designated as type Ⅲ that is not used in any other cells.They express six virally encoded nuc
【机 构】
：
Microbiology and Tumor Biology Center Karolinska Institute Sweden
【出 处】
：
BIT‘s2nd Annual World Cancer Congess-2009 (2009第二届癌症大会)
论文部分内容阅读
EBV is one of the most highly transforming viruses known.The EBV-immortalized B-cells use a viral program, designated as type Ⅲ that is not used in any other cells.They express six virally encoded nuclear proteins (EBNA1-6) and three membrane proteins (LMP1, LMP2A and B).The mechanism of transformation is not understood in detail, but it is clear that the virus exploits the normal signaling pathways of the B lymphocyte.The six growth transformation associated viral proteins (EBNA-1,-2,-3,-5,-6 and LMP1) promote cell proliferation and protect from apoptosis.DNA damage, oncogene activation and other types of cellular stress induce p53 dependent G1 arrest or apoptosis.Human DNA tumor viruses inactivate the p53 pathway as part of their primary strategy.Human papilloma virus encodes the E6 antigen that binds to p53 and to the cellular ubiquitin-protein ligase E3A.This binding leads to polyubiquitination of p53 and its proteasomal degradation.Large T encoded by Simian virus 40 binds to the DNA-binding domain of p53 and abolishes its functional activity.The transactivation activity of p53 is also blocked by HHV8 encoded LANA protein and the functionally corresponding ORF73 of Herpesvirus Saimiri.
其他文献
目的：探讨广西自然人群中脑膜炎奈瑟菌Y群的感染情况及免疫水平，为流脑预防提供科学依据。方法：在广西梧州市、南丹县、来宾市和全州县按年龄分层随机抽样，共调查0～82岁人群434人。调查对象采集咽拭子进行脑膜炎奈瑟菌(Nm)培养，采集血清标本用ELISA法检测Y群脑膜炎球菌多糖抗体IgG含量，以抗体浓度≥2μg/ml者为阳性。结果：调查对象中未检出Y群带菌者。抗体阳性率为4.38％，阳性者抗体几何平均
目的：评价玉溪沃森生物技术有限公司研制的吸附无细胞百白破联合疫苗(DTaP)的安全性和免疫原性。方法：在广西苍梧县选择600名3～5月龄，未接种过DTaP、无百日咳白喉破伤风病史的健康儿童进行临床研究，采用随机、盲法以及同类疫苗平行对照方法，将观察对象按2：1比例随机分别接种试验疫苗和对照疫苗(武汉所产DTaP)，在观察期内对接种对象进行安全性观察，采集接种前后血清标本，采用ELISA法测定白喉和
目的：研究复方中药益肾骨康汤对肿瘤骨转移癌患者血清BAP、PICP及ICTP表达的影响，从分子水平探讨益肾骨康汤的作用机制。方法：将符合纳入标准的肿瘤骨转移患者分入治疗组10例(中药益肾骨康汤1剂／日+帕米磷酸二钠90mg ivdrip 1次／月)，对照组17例(帕米磷酸二钠90mg ivdrip 1次/月)，3个月为1个疗程，另肿瘤无骨转移组11例。治疗前后以酶标法(ELASA)，检测血清骨代谢
[目的] 探讨寿胎丸对自然流产模型小鼠蜕膜组织Th1/Th2型细胞因子TNF-α、IFN-γ、IL-4和IL-10基因表达的影响。[方法] 设立正常妊娠与反复自然流产模型，并将反复自然流产模型小鼠随机分为4组，分别为模型组、寿胎丸低剂量组、寿胎丸中剂量组、寿胎丸高剂量组，应用原位杂交法检测孕14天各组蜕膜组织TNF-α、IFN-γ、IL-4和IL-10基因表达。[结果] TNF-α、IFN-γ、I
[目的] 探讨养精种玉汤对TA97、TA98、TA100、TA102菌株基因回变菌落的影响，对其遗传安全性进行研究。[方法] 采用直接平皿掺入技术，设养精种玉汤不同剂量对TA97、TA98、TA100、TA102系列菌株进行体外染毒，观察其回复突变菌落增长状况。[结果] 在0.8至200.0 mg/皿的不同测试浓度，+S9与-S9两种测试系统条件下，其作用的4种测试菌株的回变菌落数与溶剂对照相比均
[目的] 探讨通乳丹对KM小鼠骨髓细胞微核率的影响，对其遗传安全性进行研究。[方法] 采用不同时间点、不同剂量对KM小鼠进行体内骨髓细胞染毒，观察其致小鼠骨髓细胞染色体完整性受损及染色体分离异常等情况。[结果] 采用不同时间点取样，其骨髓细胞微核率与溶剂对照相比无明显增加，说明在时效关系上，不同时间点给药对小鼠骨髓细胞微核率无明显影响。采用不同剂量进一步染毒，其骨髓细胞微核率分别与溶剂对照相比也无
Disseminated adenovirus (AdV) infection is associated with high mortality in allogeneic stem cell transplant recipients.We have demonstrated that molecular detection of AdV in peripheral blood (PB) pr
The relevance of both minimal residual disease (MRD) and chimerism after hematopoietic stem cell transplantation (HSCT) for the detection of relapse has not yet been extensively studied.We investigate
Methotrexate (MTX) is an essential component of ALL treatment.Although the optimal dosage is still discussed, high-dose MTX is commonly used in induction consolidation in childhood ALL treatment proto
RPL41 is a small basic ribosomal protein with 25 amino acids.A functional screening revealed that downregulation of RPL41 induced cell transformation in NIH3T3 cells; deletions and downregulation of R