Introduction: Researches have found prenatal stress (PS) may cause offspring depression.We explored the role of pGLUR1 in offspring depression-like behavior following PS.Methods: Pregnant rats were assigned to Control group (CON), Middle prenatal stress group (MPS) and Late prenatal stress group (LPS).The pregnant rats of MPS and LPS group were exposed to restraint stress on days 7-13, 14-20 of pregnancy.Tail suspension test (TST)and Western-blot were seperately used to examine depression-like behavior and phosphorylated GluR1 (pGluR1) subunit expression of AMPAR in the hippocampus, striatum and frontal cortex of one month rat offspring.Results:In both male and female offspring the immobility time of TST in LPS and MPS was significantly longer (P<0.05)than CON, and the latency in MPS was significantly shorter (P<0.05).pGluR1 expression in hippocampus and frontal cortex of male rat offspring in LPS and MPS were significantly lower (P<0.05).Conclusion: pGluR1 might be involved in depression-like behavior induced by PS.