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Aim:To investigate the mechanism in Scutellarein-induced apoptosis of SAS human tongue cancer cells.Methods:Inhibitory effects of Scutellarein on SAS cells were detected by MTT assay.Cell apoptosis was analyzed by flow cytometry.Ultrastructural changes of SAS cells were observed by transmis-sion electron microscopy(TEM).Mitochondrial transmembrane potential(△ Ψm)were analyzed by JC-1 [5,5,6,6-Tetrachloro-1,1,3,3-tetraethyl-benzimidazolylcarbocyanine iodide].Western blot-ting was used to examine the expression level of Bcl-2,Bax and caspase-3 in SAS cells treated with Scutellarein.Results:Scutellarein inhibited the proliferation of SAS cells in a time and dose-dependent manner and increased the percent of apoptotic cells.The mitochondrial cristae were swollen and had vacu-olar degeneration.△ Ψm was decreasing when the concentration of scutellarein was increasing.Scu-tellarein effectively up-regulated the expression of mitochondrial Bax and caspase-3 and down-regu-lated the expression of Bcl-2.Conclusion:Scutellarein induces apoptosis of SAS human tongue cancer cells via activating mitochondrial signaling pathway.