【摘 要】
：
Objective Color processing has been intensively studied in ganglion cells of the primate retina, but in mouse correspondent knowledge is still incomplete.Opsin expression in mouse photoreceptor has di
【机 构】
：
Centre for Integrative Neuroscience (CIN) /Institute for Ophthalmic Research, University of Tübingen
【出 处】
：
中国神经科学学会第九届全国学术会议暨第五届会员代表大会
论文部分内容阅读
Objective Color processing has been intensively studied in ganglion cells of the primate retina, but in mouse correspondent knowledge is still incomplete.Opsin expression in mouse photoreceptor has distinct features such as opsin gradient and co-expression, but it is quite unknown how these features contribute to color processing.The main purpose of our study is to explore this issue.Methods We used dichromatic light stimulation (in the photopic range) of the short (S) and the medium (M) wavelength-sensitive mouse cone photoreceptors by two LED light sources.The relative contribution of S-and M-opsin to ganglion cell input was estimated by modulating the intensity of the two chromatic stimulus components sinusoidally or in steps.Using 2-photon imaging, we targeted GFP-positive ganglion cells in transgenic mice in whole-mounted retina for extracellular recordings.The mouse line we used (GFP-O; Feng et al., 2000) features sparse labeling of various morphological types of retinal ganglion cells.The morphology of the targeted cell was classified using image stacks taken after the recording.Results (1) Our data show that ON alpha ganglion cells (soma diameter > 20 μm, dendritic field size > 200 μm) received strong M-opsin but weak S-opsin input in dorsal retina, whereas in the ventral retina they received significantly stronger S-opsin input.This is consistent with the presence of a gradient in S-opsin co-expression in M-cones along dorsal-ventral axis in mouse retina.(2) Ratios of M-opsin and S-opsin input to a single alpha ganglion cell estimated by different sizes of stimuli vary in a systematic way, which could be explained by the cooperation of opsin gradient and center-surround antagonism.Alpha ganglion cells close to the transition zone of opsin gradient show clear color opponency when tested with large stimuli.(3) Opsin gradient could be observed in the receptive field of a single retinal ganglion cell.Conclusion Large scale opsin inhomogeneity like opsin gradient could lead to differential color processing on the level of individual retinal ganglion cell.
其他文献
Dopamine (DA) is an important neurotransmitter secreted from dopamine neurons.In the CNS, together with its receptors in striatum, prefrontal cortex, amygdale and many other areas, dopamine acts as a
Objective To evaluate the modulatory action of group Ⅰ metabotropic glutamate receptors (mGluRs) on descending activation ofmotoneurons (MNs) in neonatal rat spinal cord slices.Methods The intracellul
Objective It is known that chronic pain affects various higher brain functions including perception, emotion, cognition, and memory.However, few studies have been performed to examine pain-induced syn
Objective It has been revealed that peripheral persistent nociception results in spatial and temporal synaptic plasticity in rat hippocampal formation (HF), however, the underlying mechanisms are stil
目的 探讨环氧合酶-2在脂多糖(LPS)诱导的大鼠脊髓背角C-纤维诱发电位长时程增强(LTP)中的作用。方法 细胞外记录技术在脊髓腰膨大部记录背角浅层神经元C-纤维诱发电位。结果 (1)腹腔注射LPS后4h,强直电刺激诱导的脊髓背角LTP显著增强。(2)腹腔注射LPS后12 h或24 h,强直电刺激诱导的脊髓背角LTP与溶剂对照组比无显著差异。(3)腹腔先注射环氧合酶-2特异性抑制剂NS398,然
Objective Growing evidences suggest that electroacupuncture (EA) can improve traumatic stress-induced immuno-depression, but the mechanism needs further study.In the present study, the contribution of
By sustained activation of opioid receptors, chronic opioids cause analgesic tolerance, physical dependence, and opioid addiction, for which an effective treatment is still lacking.Amygdala, as a part
Ischemia elicits rapid release of various amino acids including glycine, which is the co-agonist of NMDA receptor (NMDAR) and one of main inhibitory neurotransmitters in central nervous system.Althoug
Objective The study aimed to explore a specific marker to label cerebrospinal fluid-contacting neurons (CSF-CNs) in rat and provide a new way to identify CSF-CNs.Methods Adult Sprague-Dawley rats, mal
Objective The dopamine (DA) system in the retina is critical to normal visual development as lack of retinal DA signalling may contribute to myopic development.The involvement of DA in myopic developm