【摘 要】
：
Background The aim of this study was to identify differentially-expressed genes (DEGs) in the growth plate of broiler chickens associated with tibial dyschondroplasia (TD) induced by thiram.RNA was ex
【机 构】
：
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070 China;College
【出 处】
：
中国畜牧兽医学会第三届中国兽医临床大会
论文部分内容阅读
Background The aim of this study was to identify differentially-expressed genes (DEGs) in the growth plate of broiler chickens associated with tibial dyschondroplasia (TD) induced by thiram.RNA was extracted from the growth plates of chickens that consumed control or thiram diets for 1,2,and 6 days.Total RNA were analyzed using eighteen chicken Affymetrix GeneChip assays to identify DEGs.The identified DEGs were also verified by real-time reverse-transcription polymerase chain reaction (RT-PCR).Results The microanalyses identified 880 DEGs,with 108,385,and 468 DEGs exhibiting at least 1.5-fold changes at days 1,2,and 6,respectively.Seven representative DEGs were further verified by real-time RT-PCR and confirmed to be upregulated in TD chickens,including lysyl oxidase (lox),heat shock protein 25 (hsp25),inhibitor of DNA binding 1 (id-1),kinectin 1 (kntl),secreted frizzled-related protein 4 (sfrp4),cadherin 1 (cdh 1),and enolase 2 (eno2).Conclusion Our findings provide a reliable bioinformatics basis for better understanding the molecular pathogenesis of TD.
其他文献
本文报道了一起湖羊由于感染枸橼酸杆菌而发生肺炎的病例.病原培养分离：选择临床症状典型的病例，死亡后无菌采取肝、脾、肺、心、肾、淋巴结、乳房等组织，接种培养基后，置37℃恒温箱中培养观察.其培养特征：在鲜血琼脂平板、普通琼脂平板、普通肉汤、葡萄糖肉汤培养基中生长.在S S琼脂平板生长显红色菌落，产生H2S.在麦康凯琼脂平板显红色菌落.呈需氧或兼厌氧生长.生化反应：病菌能发酵葡萄糖、甘露醇，并通常产气
(Introduction) Laminitis is defined as a diffuse aseptic inflammationof the lamina in the hooves of cattle,horses and sheep.Clinical laminitis is one of the most common diseases in dairy cows and caus
血小板减少是由于血小板生成减少、血小板破坏和消耗增加所致。在兽医临床上，血小板破坏和消耗增加所致的血小板减少病例较为常见，但由于诊断手段所限，血小板生成减少性疾病往往难以确诊。笔者在临床上对4例血小板降低的病例进行了跟踪治疗和观察，病例一为突然性鼻腔出血，经血常规检查白细胞升高，血小板降低，针对急性失血合并感染对患犬进行治疗，痊愈；病例二经连续的治疗、血液检查，并结合临床症状，诊断为再生障碍性贫血
用“主动免疫增强剂”和犬瘟热、犬细小病毒二联活疫苗协同免疫6周龄比格犬,研究其对犬疫苗免疫效果及外周血淋巴细胞转化能力的影响.将24只健康比格犬随机分成4组(Ⅰ～Ⅳ组),每组雌雄各半,其中Ⅰ组为疫苗对照组,Ⅱ组(低剂量组)、Ⅲ组(中剂量组),Ⅳ组(高剂量组)为试验组,分别用ELISA方法和MTT法检测各组犬免疫前及首免后第7d、14d、28 d、45 d血清中CPV抗体水平、外周血T淋巴细胞转化率
Magnolol,a hydroxylated biphenyl compound isolated from Magnolia officinalis has been reported to have anti-inflammatory properties.The purpose of this study was to evaluate the effect of magnolol on
在对中医药抗病原体最低有效浓度普遍太高，而其对机体免疫功能调节及其诱导干扰素生成的结果又与临床脱节等困惑进行综述分析的基础上，采用复杂性科学“非线性理论”对病原微生物感染多因素综合作用、辨证施治与复杂性科学“初始条件”等进行了探讨，认为中医药乃至整个生物医药抗感染研究都要转变科学观念，以复杂性科学为指导，重视各种因素的综合作用及其各自作用的初始条件；中医药辨证施治(状态分析与处理)实质上就是一种多
本研究克隆表达流行性乙型脑炎病毒E-Ⅲ抗原域蛋白将其作为包被抗原,建立了间接ELISA检测方法.试验的最佳反应条件为：最佳抗原浓度度为17.5ug/ml,血清稀释度为1∶4,封闭液用含0.5％BSA的PBST,酶标二抗稀释度为1∶5000,稀释液用含0.5％BSA的PBST,阴阳性临界值判定标准为OD630=0.30.采用该方法对127份临床样品进行检测,结果显示与商品试剂盒检测结果比较符合率达到
stevioside,a diterpene glycoside isolated from stevia rebaudiana,has been reported to have anti-inflammatory properties.however,the underlying molecular mechanisms are not well understood.the objectiv
Leptospira binds to host extracellular matrix (ECM) through surface exposed outer membrane proteins called adhesin in order to initiate infection.Of various adhesins present on the surface of the spir
Bone marrow mesenchymal stem cells (BMSCs) have shown the properties of immunosuppression and low immunogenicity and the potential capacity for differentiating into pancreatic islet-like cell clusters