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Long noncoding RNA has been proven to be involved in many biological processes in ovarian cancer (OC).However,the mechanism still remains unknown.In our study,we screened significantly downregulated NBAT-Ⅰ in two independent datasets (GSE18520 and GSE38666) from GEO (gene expression omnibus).NBAT-Ⅰ was obviously downregulated in OC tissue compared to normal counterparts (P<0.0001) and the detected levels of NBAT-Ⅰ were associated with International Federation of Gynecology and Obstetrics (FIGO) stage and tumor size guidelines.It has been shown that lower levels of NBAT-Ⅰ predict poor outcomes in OC.To investigate the functional role of NBAT-Ⅰ,pCDNA-NBAT-Ⅰ and empty vector were transfected into TOVI12D and OVCAR-3 cell lines.Overexpressed NBAT-Ⅰ significantly inhibited cell proliferation,invasion,and migration in both TOVI12D and OVCAR-3 cell lines.Finally,western blot assay indicated that NBAT-I may exert its function by targeting the ERK1/2 and AKT signaling pathways.In conclusion,our study suggests that NBAT-Ⅰ acts as an anti-oncogene in OC development.