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Mitogen-activated protein kinase (MAPK) pathways have been implicated in signal transduction of both biotic and abiotic stresses in plants.In this study,we found that the transcript of a rice (Oryza sativa)MAPKK (OsMKK1) was markedly increased by salt stress.By examining the survival rate and Na+ content in shoot,we found that OsMKK1-knockout (osmkk1) mutant was more sensitive to salt stress than the wild type.OsMKK1 activity in the wild-type seedlings and protoplasts was increased by salt stress.