Background: Rheumatoid arthritis (RA) is a chronic autoimmune disorder that primarily involves in joints.Our previous study suggested that sufficient lymphatic drainage was favorable for the treatment of RA and treatment targeting joint lymphatic drainage function contributed to the improvement of chronic inflammation of joints.To explore the effect and effect target of JuanBi Tang (JBT) on RA, we researched both on TNF transgenic (Tg) mice and Tg (fli1:egfp;gata1:dsred) zebrafish.Methods: Three-month old TNF-Tg mice and WT littermates were detected with Indocyanine green near-infrared (ICG-NIR) lymphatic imaging system before and after accepting JBT for 12 weeks.All ankle joints were assessed for hematoxylin and eosin (H&E) staining, Alcian blue/orange G (ABHO) staining, Tartrate resistant acid phosphatase (TRAP) staining and immunofluorescence staining.We add different concentration of JBT in embryo water for 48 hours after Vascular Endothelial Growth Factor Receptor 3(VEGFR-3) inhibitors stimulating 2-day-post-fecundation (2dpf) Tg zebrafish for 6 hours.The length of lymphatic thoracic duct of each Zebrafish would be measured with laser scanning confocal microscopy.Results: JBT could increase TNF-tg mice astragalus bone area (1.54 ± 0.78mm2) in contrast to saline mice (0.75 ± 0.24 mm2, P<0.05), almost equal to WT mice (1.58 ± 0.75 mm2, P>0.05), While could reduce the inflammation area (1.07 ± 0.44 mm2, P<0.05) of TNF-tg mice (1.55 ± 0.48mm2).Cartilage area (1.77 ± 0.44mm2, P<0.05) increased more than 20 folds in JBT group compared with saline group.Increased TRAP+ area and impaired lymphatic function of TNF-Tg mice were also rescued by JBT (P<0.05).JBT could promote the formation of Tg zebrafish lymphatic thoracic duct (P<0.05), which inhibited by VEGFR-3 inhibitors.Conclusions: JBT decreased synovial inflammation, bone and cartilage erosion while increased the ICG clearance at of TNF-Tg mice,implying that JBT is a promising agent for treating RA through its promoting lymphatic drainage function effects.