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Miniaturization of chromatographic separation systems provides a means of greatly increasing sensitivity in LC-MS.Here we demonstrate the use of an integrated microfluidic device for the LC/MS/MS investigation of the in vitro microsomal metabolism of the model drug propranolol using a sample volume of 1 μL of a 1 μMol incubation.The system was capable of obtaining high quality MS and MS/MS data from the injection of test drug substance containing sufficient information to correctly derive the structure of the drug metabolites.These data study suggest that these types of micro LC/MS/MS device are robust enough for routine applications, and well suited to the analysis of small samples.