SMALL INTERFERING RNA TRANSFECTION IN CULTURED CARDIAC FIBROBLASTS

来源 :第二十四届长城国际心脏病学会议、亚太心脏大会暨国际心血管病预防与康复会议(The 24th Great Wall Int | 被引量 : 0次 | 上传用户:wangyongwangyongwang
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Objectives Small interfering RNA (siRNA) methodology suppresses specific gene expression, thus mimicking loss-of-function mutation and enabling in vitro and in vivo gene function analysis.siRNA technology is also a promising tool for gene therapy of various diseases.Lipofectamine RNAiMAX, a new transfection reagent, has been confirmed high efficiency in deliveringsiRNA into mesenchymal stem cells and coronary vascular smooth muscle cells.
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