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TMV, CMV, TEV and PVY are great threats to tobacco production in China.To find out the distribution and molecular variation of these tobacco viruses, we conducted an investigation a-nd analysis of tobacco viruses diseases in 12 main tobacco production areas in China.409 (154 in 2010 and 255 in 2011) tobacco leaf samples were collected from 12 main production areas in Chi-na.All the samples were tested by DAS-ELISA and RT-PCR.