【摘 要】
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Objects: Explore a novel PCR-RFLP method for identifying the single-nucleotide polymorphism (SNP) of rs 1048943 of CYP1A 1 gene.Methods: The study used an improved PCR-RFLP method with mismatched base
【机 构】
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Department of Occupational Health and Occupational Disease, College of Public Health,Zhengzhou Unive
【出 处】
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第六届中国毒理学会免疫毒理专业委员会换届及学术会议
论文部分内容阅读
Objects: Explore a novel PCR-RFLP method for identifying the single-nucleotide polymorphism (SNP) of rs 1048943 of CYP1A 1 gene.Methods: The study used an improved PCR-RFLP method with mismatched base for detection of the single nucleotide polymorphism rs1048943.In the reverse primer, a deoxynucleotide A was substituted with C, and after PCR a new restriction enzyme site for StyⅠ was introduced into the PCR products.A total of 320 samples from Han Chinese were tested to evaluate this novel method.Results: After detecting 320 Chinese Han individuals, the genotype frequencies were 63.74% for AA, 31.54% for AG, and 4.72% for GG.The allelic frequencies were 75.48% for A and 24.52% for G.The x2 test showed the genotype and allele frequencies of CYP1A1 do not deviate from Hardy-Weinberg equilibrium, and the sequences of amplified products were consistent with the one published in Genbank with the exception of mismatched base.Conclusions: Based on PCR with mismatched primers we designed, the CYP1A1 polymorphism could be identified effectively with low cost.
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