Effects of different pulse fed-batch methods on production of the anti-cancer compound 1403C by marine mangrove endophytic fungus Halorosellinia sp.(No.1403) in a 5-L bioreactor were investigated.Since high glucose concentrations improved mycelial growth but inhibited 1403C production,the cultures were pulse fed with glucose solutions to keep the residual glucose lower than 4 g/L but higher than 0.5 g/L during rapid growth phase (0-50 h).